Bonzanni M, Payne SL, Adelfio M, Kaplan DL, Levin M, Oudin MJ, 2020 · passages 30 to 33 of 34

Defined extracellular ionic solutions to study and manipulate the cellular resting membrane potential

Generation and use of the calibration curve

Where F is the fluorescence measurement, m is the slope, V is the voltage measurement and q is the intercept.

In order to calculate the voltage change upon application of two solutions (ΔV), we solve the following system of equations:

For any given change in the fluorescence (ΔF) readout in two different conditions, through Eqn 3 is possible to calculate the relative voltage change (ΔV). For instance, if we want to compute ΔV for C1 and C2, Eqn 3 can be written as:

Statistics

Patch clamp data were analyzed with Clampfit10 (Axon) and Origin Pro 9. Linear fitting was performed reporting the adjusted R2 value. Mean±s.e.m. was reported from at least two different independent experiments for all the presented data. Either the number of cells or ROIs and the number of experiments were reported for each dataset.